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a nebnext ultra ii dna library prep kit for illumina  (New England Biolabs)


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    Structured Review

    New England Biolabs a nebnext ultra ii dna library prep kit for illumina
    A Nebnext Ultra Ii Dna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+ultra+ii+dna+library+prep+kit/NEBNext+Ultra+II+FS+DNA+Lib+Prep+Kit/pmc13091997-144-6-7
    Average 99 stars, based on 2840 article reviews
    a nebnext ultra ii dna library prep kit for illumina - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Dual-functional hydrogel platform suppresses M1 activation and stabilizes M2 macrophages in intervertebral disc degeneration
    Article Snippet: III) The enriched mRNA was reverse-transcribed into double-stranded complementary DNA (cDNA) using the PrimeScript RT reagent Kit (Takara, Japan). .. IV) The cDNA ends were repaired, and the sequencing library was constructed by PCR amplification with adapter ligation using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). .. V) Sequencing was performed on the Illumina NovaSeq 6000 platform by Gene Denovo Biotechnology Co., Ltd (Guangzhou, China), generating 150 bp paired-end reads.

    Article Title: Whole-genome sequence dataset of Pasteurella multocida strain EH32 isolated from buffaloes in Vietnam
    Article Snippet: .. Data collection , Genomic DNA of P. multocida strain EH32 was extracted from pure cultures using the QIAamp DNA Mini Kit. Sequencing libraries were prepared with the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit. Libraries were sequenced using a paired-end (2 × 150 bp) chemistry on the PacBio Onso platform. Genome annotation was performed with Prokka v1.14.6, and functional categories were assigned using the COG and KEGG databases; carbohydrate-active enzymes were identified with dbCAN3.. ..

    Article Title: Ketone body metabolism activates the immune response against Staphylococcus aureus infection by fueling the tricarboxylic acid cycle and affecting histone β-hydroxybutyrylation
    Article Snippet: For RNA sequencing (RNA-seq), high-quality total RNA was amplified using the SMARTer Ultra Low Input RNA Kit for Sequencing v2 (Takara Bio). .. The resulting cDNA was converted into sequencing libraries using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA). .. Libraries were quantified, pooled in equimolar ratios, and sequenced on an Illumina HiSeq 2500 platform (Illumina, San Diego, CA, USA), generating 25-60 million single-end reads (50 bp or 75 bp) per library.

    Construct:

    Article Title: Dual-functional hydrogel platform suppresses M1 activation and stabilizes M2 macrophages in intervertebral disc degeneration
    Article Snippet: III) The enriched mRNA was reverse-transcribed into double-stranded complementary DNA (cDNA) using the PrimeScript RT reagent Kit (Takara, Japan). .. IV) The cDNA ends were repaired, and the sequencing library was constructed by PCR amplification with adapter ligation using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). .. V) Sequencing was performed on the Illumina NovaSeq 6000 platform by Gene Denovo Biotechnology Co., Ltd (Guangzhou, China), generating 150 bp paired-end reads.

    Article Title: A tunable CTD grammar governs the spatial programming of the transcription cycle.
    Article Snippet: .. ChIPseq libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit and sequenced using an Illumina NovaSeq 6000 system by Novogene. ..

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing
    Article Snippet: .. Alternatives: This protocol uses NEBNext Ultra II DNA Library Prep Kit with Sample Purification Beads (NEB #E7103S) to construct library. ..

    Polymerase Chain Reaction:

    Article Title: Dual-functional hydrogel platform suppresses M1 activation and stabilizes M2 macrophages in intervertebral disc degeneration
    Article Snippet: III) The enriched mRNA was reverse-transcribed into double-stranded complementary DNA (cDNA) using the PrimeScript RT reagent Kit (Takara, Japan). .. IV) The cDNA ends were repaired, and the sequencing library was constructed by PCR amplification with adapter ligation using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). .. V) Sequencing was performed on the Illumina NovaSeq 6000 platform by Gene Denovo Biotechnology Co., Ltd (Guangzhou, China), generating 150 bp paired-end reads.

    Amplification:

    Article Title: Dual-functional hydrogel platform suppresses M1 activation and stabilizes M2 macrophages in intervertebral disc degeneration
    Article Snippet: III) The enriched mRNA was reverse-transcribed into double-stranded complementary DNA (cDNA) using the PrimeScript RT reagent Kit (Takara, Japan). .. IV) The cDNA ends were repaired, and the sequencing library was constructed by PCR amplification with adapter ligation using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). .. V) Sequencing was performed on the Illumina NovaSeq 6000 platform by Gene Denovo Biotechnology Co., Ltd (Guangzhou, China), generating 150 bp paired-end reads.

    Adapter Ligation:

    Article Title: Dual-functional hydrogel platform suppresses M1 activation and stabilizes M2 macrophages in intervertebral disc degeneration
    Article Snippet: III) The enriched mRNA was reverse-transcribed into double-stranded complementary DNA (cDNA) using the PrimeScript RT reagent Kit (Takara, Japan). .. IV) The cDNA ends were repaired, and the sequencing library was constructed by PCR amplification with adapter ligation using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). .. V) Sequencing was performed on the Illumina NovaSeq 6000 platform by Gene Denovo Biotechnology Co., Ltd (Guangzhou, China), generating 150 bp paired-end reads.

    Functional Assay:

    Article Title: Whole-genome sequence dataset of Pasteurella multocida strain EH32 isolated from buffaloes in Vietnam
    Article Snippet: .. Data collection , Genomic DNA of P. multocida strain EH32 was extracted from pure cultures using the QIAamp DNA Mini Kit. Sequencing libraries were prepared with the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit. Libraries were sequenced using a paired-end (2 × 150 bp) chemistry on the PacBio Onso platform. Genome annotation was performed with Prokka v1.14.6, and functional categories were assigned using the COG and KEGG databases; carbohydrate-active enzymes were identified with dbCAN3.. ..

    Sample Purification:

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing
    Article Snippet: .. Alternatives: This protocol uses NEBNext Ultra II DNA Library Prep Kit with Sample Purification Beads (NEB #E7103S) to construct library. ..



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    Image Search Results


    Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit. The workflow includes. (1) end repair and dA-tailing of fragmented genomic DNA. (2) adaptor ligation (hairpin adaptor containing dU); USER enzyme cleavage to open the adaptor, and (3) PCR enrichment with indexed primers to generate the final sequencing library.

    Journal: STAR Protocols

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

    doi: 10.1016/j.xpro.2026.104513

    Figure Lengend Snippet: Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit. The workflow includes. (1) end repair and dA-tailing of fragmented genomic DNA. (2) adaptor ligation (hairpin adaptor containing dU); USER enzyme cleavage to open the adaptor, and (3) PCR enrichment with indexed primers to generate the final sequencing library.

    Article Snippet: Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit.

    Techniques: Ligation, Sequencing